Biofunctionalization of Gold Nanorods: A Comparative Study on Conjugation Methods for Fabrication of Nanobiosensors
Saeede
Ranjbari Baglou
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
author
Tahereh
Tohidi Moghadam
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
author
Bijan
Ranjbar
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran
author
text
article
2019
eng
Gold nanoparticles are promising materials for biomedical applications because of the attractive optical properties such as the absorption and scattering of light at resonant wavelength. Due to the fruitful applications of gold nanoparticles (GNPs), they are appropriate for a variety of biological studies. One of the most important applications of nanoparticles which consist of transport of therapeutically relevant protein to in vivo or in vitro targets is protein carriers. Protein folding and properties of probable intermediates during the folding of proteins had been investigated in several studies. The molten globule state, a main intermediate of protein folding, has native-like secondary but perturbation of tertiary structure. Consequently, the influence of gold nanoparticles concentration on a model protein was studied at the buffer concentration by far- and near-UV circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), intrinsic fluorescence emission spectroscopy and 8-Anilino-1-naphthalenesulfonic acid binding. The results indicated that the interactions between gold nanoparticles and lysozyme lead to the formation of molten globule-like state.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
23
34
https://www.bmmj.org/article_38128_93bf9a5d2bd27106040cd90722af88e6.pdf
TALEN: A Tool for Genome Editing
SeiedAbdolmajid
Angaji
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
Behnaz
Beikzadeh
Department of cell and molecular biology, Faculty of biological sciences, Kharazmi university, Tehran, Iran
author
text
article
2019
eng
Genome editing by engineered nuclease enzymes, which, induce DNA double strand break (DSB) followed by the activation of repair mechanisms, is of great importance to researchers in the field of biology and medicine. TALEN (Activator Transistor like Effector Nuclease) as a tool for genome editing is widely used in a variety of organisms and cells to study the role of genes or mutations in biological or pathological pathways, create model organisms or a therapeutic approach to single genetic disorders such as sickle cell anemia. TALE consists of: 1) N-terminal T3S signal, 2) Central tandem repeat domain, 3) C-terminal nuclear localization signal (NLS) and 4) Acidic transcriptional activation domain (AD, fused to a non-specific domain for DNA double strand break). DSB can undergo changes such as insertion, deletion, inversion, translocation through two major repair pathways of NHEJ and HDR. In this review article, we will first discuss the TALEN technology, assembly of custom TALEN and its transmission techniques. This is followed by studies on genome editing, various repair mechanisms and donor design in the HDR pathway. Finally, the applications of this technology will be discussed.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
1
11
https://www.bmmj.org/article_36637_7eb7260eea4f2d29f1542af37abf70e3.pdf
The Effect of Nerol on Behavioral, Biochemical and Histological Parameters in Male Wistar Alzheimer's Rats
Sajjad
Ghashghaei
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Maryam
Ghobeh
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Parichehreh
Yaghmaei
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2019
eng
One of the most important reasons for dementia is Alzheimer's disease (AD) characterized by amyloid plaques and neuronal death. Using natural therapies has always been a great concern for AD. Herein, nerol, as a natural monoterpene, was applied to examine its protective and therapeutic effects on a rat model of AD. Fifty six Wistar, male rats were randomly assigned to 7 groups (n= 8 rats/group), including control group with no Aβ42 injections, group 2 with Aβ42 injection into rats’ hippocampus, group 3 with injection of phosphate buffer saline, as Aβ buffer, into rats’ hippocampus, group 4, 5, and 6 that received nerol at 30, 60, and 90 mg/kg, respectively, after being injected with Aβ42, and group 7 that received nerol (60 mg/kg) in a protective mode before Aβ injection. Behavioral, biochemical, and histological parameters were evaluated in all groups. Alzheimer’s-induced group showed impairment in lipid profile, antioxidant enzyme level, long-term memory, enzymes activity along with loss of neuronal cells. Treatment with nerol improved the lipid profile, antioxidant enzyme level, number of amyloid plaques, and memory function. Protection with nerol (60 mg/kg) also demonstrated similar improvements against AD. Hence, nerol has shown capability of improving the deficiency of neuronal cells, memory as well as antioxidant enzyme level associated with AD.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
12
22
https://www.bmmj.org/article_37221_ee23ac1808c85d77a6e561281f816a2d.pdf
Expression and Purification of Firefly Luciferase and its Interaction with Cadmium Telluride Quantum Dot
Sadegh
Zomorodimanesh
Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Saman
Hosseinkhani
Tarbiat Modares University
author
Hadi
Baharifar
Department of Medical Nanotechnology, Applied Biophotonic Research center, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Farzad
Yousefi
Co-funder & CEO Nora gene pishro,knowledge base company,Tehran, Iran
author
Janet
Farsad
Miniistry of Health_Environmental and Occupational health center, Tehran, Iran
Abstract
author
text
article
2019
eng
Firefly luciferase is a monomeric enzyme of 62 kDa that catalyzes emission of green to yellow region, typically 550–570 nm upon reaction with d-luciferin, ATP, and molecular oxygen. Semiconductor nanocrystal, also known as the quantum dots (QDs), are nanoscaled inorganic particles in size range of 1-10nm. QDs have properties, such as sharp and symmetrical emission spectra, size-dependent emission, good chemical and photostability and high quantum yield. In this study, recombinant P. pyralis luciferase was expressd and then purified based on N -terminal His-tag. Then the effects of the Cadmium Tellurium QD(6.2nm) on the tertiary structure, kinetic properties, Bioluminescence Resonance Energy Transfer, thermal stability and remaining activity of luciferase was assayed using fluorescence spectroscopy and bioluminescence assay. The results showed that the CdTe QD affects the tertiary structure of the luciferase enzyme. The kinetic parameters of the enzyme also changed, as well as the thermal stability. The remaining activity was decrease in the presence of quantum dot compared to the native enzyme and BRET also was not observed in the presence of QD.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
35
46
https://www.bmmj.org/article_38156_8e84d051b6da76e952e29c07d0d5554a.pdf
Elucidating the Anti-aging Ursolic Acid through Evaluation of SIRT1 and SIRT6 in the Mice Skeletal Muscle Tissue
Sahar
Ahmadzadeh
Department of Biochemistry, Faculty of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
author
Ali Reza
Noori
Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
author
Nuredin
Bakhtiari
Department of Biochemistry, Faculty of Biological Science, Young Researchers Club, North- Tehran Branch, Islamic Azad University, Tehran, Iran.
author
Roohullah
Hemmati
Department of Biology, Faculty of Basic Science, Shahrekord University, Shahrekord, Iran.
author
text
article
2020
eng
The process of skeletal muscle aging is linked to the development of age-related diseases such as metabolic syndrome, diabetes, cancer and Alzheimer’s disease. Hence, to further investigate the effect of Ursolic Acid (UA) endorses previous studies on skeletal muscle rejuvenation, we tested whether UA alters two prominent anti-aging biomarkers such as SIRT1 and SIRT6 in skeletal muscle. UA is a triterpenoid compound widely found in natural plants and fruits, with known effects on skeletal muscle. To conduct this experiment, UA dissolved in Corn Oil (200mg/ml) was administrated (200mg/kg IP injection) twice daily for 7 days to male aged-mice C57BL/6. Then, mice skeletal muscle tissues were isolated and proteins were examined through immunofluorescence microscopy. Our findings clearly illustrated that UA enhances SIRT1 (~3.5 folds) and SIRT6 (~4 folds) expression levels, p
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2020
47
57
https://www.bmmj.org/article_38182_1f83dec7e063477e458bc5e43258ffac.pdf
Dual effect of Caffeine and Curcumin as Antioxidants on Human Hemoglobin in the Presence of Methyl Tert-butyl Ether (MTBE)
Ismaeil Hossein
Najdegerami
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
author
Ghader
Hosseinzadeh
Department of Chemical Engineering, University of Bonab, Bonab, Iran
author
Vahid
Sheikh-Hasani
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
author
Faezeh
Moosavi-Movahedi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran
author
Parvaneh
Maghami
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Nader
Sheibani
Departments of Ophthalmology and Visual Sciences, Cell and Regenerative Biology, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
author
Ali Akbar
Moosavi-Movahedi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran
author
text
article
2019
eng
Extensive use of methyl tert‐butyl ether (MTBE) has raised significant threats to the environment through pollution of environmental resources including ground waters. This compound could accumulate in the blood stream through inhalation of contaminated air since MTBE has a high affinity for blood proteins. The interaction of blood proteins such as human hemoglobin (Hb) with MTBE results in conformational and likely functional changes. The main mechanism for harmful effects of MTBE on Hb is through production of reactive oxygen species (ROS). In this regard, the present work was proposed to study the possible antioxidant potential of two dietary antioxidant agents, curcumin and caffeine, on the reduction of MTBE damage on Hb. Different spectroscopic methods including fluorescence, UV‐visible, circular dichroism, chemiluminescence, and molecular docking were used to study the interactions of curcumin and caffeine with Hb in the presence of MTBE. Our results showed caffeine could decrease the aggregation and ROS effects of MTBE on Hb. However, in the presence of curcumin the MTBE mediated aggregation of Hb was enhanced. These opposing effects of curcumin and caffeine as antioxidants were mainly contributed to the high iron chelating activity of curcumin. Thus, the complex formation between curcumin and heme further enhanced ROS production capability of MTBE.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
58
71
https://www.bmmj.org/article_38958_28d1d8dd2ad7a296276f7ab9ebaf7501.pdf
A Comparative Kinetic Study of the Photo-oxidation of Phenol by Tetra-cationic Porphyrins and Zinc Compounds
Fatemeh
Fayyaz
Department of Chemistry, Payame Noor University (PNU), P. O. Box: 19395-3697 Tehran, Iran
author
Rahmatollah
Rahimi
Bioinorganic Chemistry Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
author
Ali
Maleki
Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran
author
text
article
2019
eng
Kinetic of the photo-oxidation of phenol in aqueous alkaline and neutral buffer solutions was studied and compared by irradiation of visible light in the presence of tetra-cationic porphyrins and their zinc metal ion photo-sensitizers. The porphyrins 5,10,15,20-tetrakis(3-methylpyridinium)porphyrin (TMPyP(3)); 5,10,15,20-tetrakis(4-methylpyridinium)porphyrin (TMPyP(4)); 5,10,15,20-tetrakis(4-N,N,N-trimethylanilinium)porphyrin (TAPP) and their zinc ion complexes were used in this study. In the dark conditions, none of the porphyrin compounds showed any effect on the reducing of phenol. The results showed that the rate of the photo-oxidation of phenol in alkaline medium has been increased in the presence of these photo-sensitizers. Among them, the rate of photo-oxidation by TAPP was more than the other ones. In neutral medium, the rate of the photo-oxidation of all of the compounds have been shown approximately similar behavior. It was found that the dominant pathway of phenol degradation occurred in the reaction with molecular singlet oxygen (1O2). Kinetic parameters of the phenol reaction with 1O2 were estimated. According to the equation (ln A/A_0 =k_obs t) and by plotting ln A/A_0 against time, the rate of the reaction was obtained first order.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
72
82
https://www.bmmj.org/article_38959_65380ff27f5566990e828df853f8e4b5.pdf
Apoptosis Induction by Calystegia Sepium HPLC Fraction can be via Dual Targeting of MDM2 and BCL-2 in Cancer Cells
Mohammad Hossein
Rezadoost
Ph.D., Plant Biotechnology Dep., Faculty of Agricultural sciences, University of Guilan, Rasht, 4199613776, Iran
author
Alireza
Ghasempour
Ph.D., Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran
author
Hassan
Hassani Kumleh
Ph.D., Plant Biotechnology Dep., Faculty of Agricultural sciences, University of Guilan, Rasht, 4199613776, Iran
author
text
article
2019
eng
In this research the different extracts of plants were tested to find the special fraction of optimum extract that induce the apoptosis. Cytotoxicity of Methanolic leaf extracts of Catharanthus roseus, Calystegia sepium, Ethanolic leaf extract of Tradescantia pallida and Nasturtium officinale and the Ethanolic and Methanolic bulb extract of Narcissus tazetta treatment after 24 h exposure was investigated using MTT assay and flow cytometric cell cycle and apoptosis tests in HeLa and G292 cancer cells and HGF-1 normal cell. The most effective extract was separated into fractions by preparative HPLC. Fractions cytotoxicity and their effects on some genes expression involved in apoptosis were determined by Real-Time PCR. Results indicated that C5 fraction of Calystegia sepium leaf Methanolic extract created the strong cytotoxic effect on cancer cells when compared to doxorubicin as the standard drugs. In addition, C5 fraction increased the expression level of inflammatory transcription factor like nuclear-factor-KB1, cell cycle inhibitors like TP53 dependent G2 arrest mediator (RPRM) and Cyclin dependent kinase inhibitor 1A (CDKN1A, p21). In contrast, C5 fraction decreased the expression level of MDM2 and BCL-2 that dual targeting of both can consider as a therapeutic strategy. Thus, Calystegia sepium has a lot of potential to be introduced as a new anticancer plant.
Biomacromolecular Journal
Iran Society of Biophysical Chemistry (ISOBC)
7280-2423
5
v.
1
no.
2019
83
95
https://www.bmmj.org/article_39767_79cfceb41aaeab0bc8f668730c173755.pdf