@article { author = {Assari, Neda and Angaji, SeiedAbdolmajid and Morovvati, Saeed}, title = {Association of Polymorphism at 3׳-UTR of Urokinase Gene with Risk of Calcium Kidney Stones}, journal = {Biomacromolecular Journal}, volume = {2}, number = {1}, pages = {21-27}, year = {2016}, publisher = {Iran Society of Biophysical Chemistry (ISOBC)}, issn = {7280-2423}, eissn = {}, doi = {}, abstract = {Urokinase might play a role in the formation of kidney stones. This study was done to determine the association between +4065 T/C polymorphism at the 3′-untranslated region of urokinase gene and calcium kidney stones. This Case-Control study was carried out on 70 cases with a history of calcium kidney stones and 70 controls from the Baqiyatallah hospital of Tehran in 2013. The study of polymorphism was performed by Allele-specific PCR method. Mutant T allele frequency was %41 in cases and %18 in controls. Hardy-Weinberg equilibrium (HWE) was tested using chi-square test. The chi-square showed that the control population is in HWE, and as expected, the patient group does not follow HWE. The odds ratio for the risk of the T allele was 1.7 (%95 confidence interval 0.2988-1.158). A significant association was found between the urokinase gene T/C polymorphism and the formation of calcium kidney stones. Also, effect modification was examined for both sexes and different age groups. According to the results, T polymorphism in the 3’-UTR of urokinase gene may increase with age.}, keywords = {single nucleotide polymorphism,3'-Untranslated region,AS-PCR,Hardy-Weinberg equilibrium}, url = {https://www.bmmj.org/article_21157.html}, eprint = {https://www.bmmj.org/article_21157_4a8b1d25ff62021c47a0121c1d1bfecb.pdf} }