TY - JOUR ID - 34368 TI - Antibacterial and Anti-biofilm Investigation of Electrospun PCL/gelatin/Lawsone Nano Fiber Scaffolds against Biofilm Producing Bacteria JO - Biomacromolecular Journal JA - BMMJ LA - en SN - 7280-2423 AU - Adeli-Sardou, Mahboubeh AU - Torkzadeh-Mahani, Masoud AU - Yaghoobi, Mohammad Mehdi AU - Dodel, Masoumeh AD - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran AD - Department of Biotechnology, Institute of Sciences, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran AD - Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran Y1 - 2018 PY - 2018 VL - 4 IS - 1 SP - 46 EP - 57 KW - Antibacterial KW - Anti-biofilm activity KW - Lawsone KW - Polycaprolactone KW - Gelatin KW - Electrospinning DO - N2 - The aim of this study was to evaluate anti bacterial and antibiofilm activity of PCL/gelatin nanofibers containing Lawsone against four pathogenic bacteria. Different concentrations of Lawsone (2-hydroxy-1, 4- naphthoquinone) (1, 3, 5 and 10%) were incorporated into polycaprolactone (PCL), gelatin (GEL) nanofibers via electrospinning technique. Presence of Lawsone in the scaffold confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial and antibiofilm activity of Lawsone loaded scaffolds against clinically isolated Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Proteus mirabilis were investigated by disc diffusion method. All scaffolds were able to inhibit the growth of S. aureus and MRSA. Additionally, PCL/GEL/Lawsone 10% had bactericidal efficacy against P. mirabilis. Obtained results of the biofilm formation assay revealed that PCL/GEL/Lawsone 10% inhibited the biofilm of S. aureus, MRSA and P. mirabilis by 56%, 32%, and 15%, respectively. Due to good antibacterial and antibiofilm activity of Lawsone containing scaffolds, PCL/GEL 10% can be a good candidate for application in wound dressing patches. UR - https://www.bmmj.org/article_34368.html L1 - https://www.bmmj.org/article_34368_cff8ced3112527178620e41b4c5aa0b3.pdf ER -