Altered Expression of Epidermal Growth Factor Receptor (EGFR) in Glioma

Document Type: Article

Author

1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran.

Abstract

      EGFR is a key molecule in cancer cells. EGFR signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. We extracted proteins from astrocytoma (III and IV) oligodendroglioma(IV) tumors and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometry method. In this study, we separated proteins by the two-dimensional gel (2DG) electrophoresis method, and the spots were analyzed and compared using statistical data and Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-TOF MS). EGFR position showed in the diagram cluster for oligodendroglioma tumors (t-test and one-way ANOVA; P < 0.05). EGFR proteins were definitely with isoelectric pH, molecular weight and protein databank detected which has an up-regulation. Proteomics analysis revealed an unexpected alteration in the expression of certain protein networks in EGFR gliomas. The EGFR dependent signaling pathways are most frequently reported in high grade oligodendroglioma and affect all histological closes. Thus EGFR could be a candidate biomarker in glioma tumors.

Graphical Abstract

Altered Expression of Epidermal Growth Factor Receptor (EGFR) in Glioma

Keywords

Main Subjects


 

 

[1]           K. Gollapalli, S. Ray, R. Srivastava, D. Renu, P. Singh, S. Dhali, J. Bajpai Dikshit, R. Srikanth, A. Moiyadi, S. Srivastava, Proteomics12 (2012) 2378.

[2]           M. Pooladi, S. Khaghani-Razi-Abad, M. Hashemi. Indian J. Cancer 51 (2014) 159.

[3]           H.F. Poon, R.A. Vaishnav, T.V. Getchell, M.L. Getchell, D.A. Butterfield, Neurobiol Aging 27 (2006) 1010.

[4]           M. Pooladi, S. Khaghani-Razi-Abad, N. Nazarian, L. Firouzi-Dalvand, M. Hooshiyar, J. Protein and Proteomics 5 (2014) 55.

[5]            B. Collet, N. Guitton, S. Saïkali, T. Avril, C. Pineau, A. Hamlat, J. Mosser, V. Quillien, Proteome Sci. 9 (2011) 16.

[6]           C. Horbinski, J. Hobbs, K. Cieply, S. Dacic, R.L. Hamilton, Am. J. Pathol. 179 (2011) 1638.

[7]           S. Pinel, J. Mriouah, M. Vandamme, A. Chateau, F. Plénat, E. Guérin, L. Taillandier, V. Bernier-Chastagner, J.L. Merlin, P. Chastagner, PLoS One 8 (2013) e68333.

[8]           J.S. Guillamo, S. de Boüard, S. Valable, L. Marteau, P. Leuraud, Y. Marie, M.F. Poupon, J.J. Parienti, E. Raymond, M. Peschanski, Clin. Cancer Res. 15 (2009) 3697.

[9]           J.J. Raizer, J. Neurooncol. 74 (2005) 77.

[10]       A. Diaz Miqueli, J. Rolff, M. Lemm, I. Fichtner, R. Perez, E. Montero,  Br J. Cancer 100 (2009) 950.

[11]       I.K. Mellinghoff, M.Y. Wang, I. Vivanco, D.A. Haas-Kogan, S. Zhu, E.Q. Dia, K.V. Lu, K. Yoshimoto, J.H.  Huang, D.J. Chute, B.L. Riggs, S. Horvath, L.M. Liau, W.K. Cavenee, P.N. Rao, R. Beroukhim, T.C. Peck, J.C. Lee, W.R. Sellers, D. Stokoe, M. Prados, T.F. Cloughesy, C. L. Sawyers, P.S. Mischel, N Engl. J. Med. 353 (2005) 2012.

[12]       T.J. Lynch, D.W. Bell, R. Sordella, S. Gurubhagavatula, R.A. Okimoto, B.W. Brannigan, P.L. Harris, S.M. Haserlat, J.G. Supko, F.G. Haluska, D.N. Louis, D.C. Christiani, J. Settleman, D.A. Haber, N Engl. J. Med. 350 (2004) 2129.

[13]       Y. Hirose, M. Kojima, M. Sagoh, T. Hayashi, H. Murakami, K. Shimazaki, K. Yoshida, T. Kawase,  Neurol. Med. Chir. (Tokyo). 38 (1998) 851.

[14]       Y. Sun, A.C. Hedman, X. Tan, R.A. Anderson,  Cell Cycle 12 (2013) 1991. 

[15]        R.T. Abdallah, J.S. Keum, H.M. El-Shewy, M.H. Lee, B. Wang, M. Gooz, D.K. Luttrell, L.M. Luttrell, A.A. Jaffa, J. Biol. Chem. 285 (2010) 35206.

[16]       F.L. Roudabush, K.L. Pierce, S. Maudsley, K.D. Khan, L.M.  Luttrell, J. Biol. Chem. 275 (2000) 22583.

[17]        H. Daub, F.U. Weiss, C. Wallasch, A. Ullrich, Nature 379 (1996) 557.

[18]       K. Imami, N. Sugiyama, H. Imamura , M. Wakabayashi, M. Tomita, M. Taniguchi, T. Ueno, M. Toi, Y. Ishihama,  Mol. Cell Proteomics 11 (2012) 1741.

[19]        C. Faulkner, A. Palmer, H. Williams, C. Wragg, H.R. Haynes, P. White, R.M. DeSouza, M. Williams, K. Hopkins, K.M. Kurian, Br J, Neurosurg. (2014) 1.

[20]        H. Duan, L. Qu, C. Shou, Cancer Cell Int. 14 (2014) 135.

[21]        M. Pooladi, M. Rezaei-Tavirani, M. Hashemi, S. Hesami-Tackallou, S. Khaghani Razi Abad, A. Moradi, A.R. Zali, M. Mousavi, A. Rakhshan, L. Firozi- dalvand, R. Omidi, Iran. J. Cancer Prevention 3 (2014) 130.

[22]        R.F. Deighton, D.M. Short, R.J. McGregor, A.J. Gow, I.R. Whittle, J. McCulloch, J. Neurosci. Methods 180 (2009) 321.

[23]       A.M. Ardekani, M.M. Akhondi, M.R. Sadeghi, Arch. Iran. Med. 11 (2008) 427.

[24]       T.C. Ramos, J. Figueredo, M. Catala, S. González, J.C. Selva, T.M. Cruz, C. Toledo, S. Silva, Y. Pestano, M. Ramos, I. Leonard, O. Torres, P. Marinello, R. Pérez, A. Lage, Cancer Biol. Ther. 5 (2006) 375.

[25]        X. Fang, C. Wang, B.M. Balgley, K. Zhao, W. Wang, F. He, R.J. Weil, C.S. Lee, J. Proteome Res. 11 (2012) 3937.

[26]        L. Ouyang, Y. Chen, X.Y. Wang, R.F. Lu, S.Y. Zhang, M. Tian, T. Xie, B. Liu, G. He, Phytomedicine 21 (2014) 1658.

[27]       H. Daub, C. Wallasch, A. Lankenau, A. Herrlich, A. Ullrich, EMBO J. 16 (1997) 7032.

[28]        B.M. Costa, M. Viana-Pereira, R. Fernandes, S. Costa, P. Linhares, R. Vaz, C. Pinheiro, J. Lima, P. Soares, A. Silva, F. Pardal, J. Amorim, R. Nabiço, R. Almeida, C. Alegria, M.M. Pires, C. Pinheiro, E. Carvalho, P. Oliveira, J.M. Lopes, R.M. Reis, Cancer Epidemiol. Biomarkers Prev. 20 (2011) 2610.

[29]        Y.H. Zhou, K.R. Hess, V.R. Raj, L. Yu, L. Liu, A.W. Yung, M.E. Linskey,  Establishment of prognostic models for astrocytic and oligodendroglial brain tumors with standardized quantification of marker gene expression and clinical variables. Biomark Insights. 5 (2010) 53-68.

[30]        T. Hide, T. Takezaki, Y. Nakatani, H. Nakamura, J. Kuratsu, T. Kondo, Stem Cells 29 (2011) 590.

[31]       J. Reifenberger, G. Reifenberger, K. Ichimura, E.E. Schmidt, W. Wechsler, V.P. Collins, Am. J. Pathol. 149 (1996) 29.

[32]        C. Horbinski, G. Wang, C.A. Wiley, Int. J. Clin. Exp. Pathol. 3 (2010) 226.

[33]        J.H. Suh, C.K. Park, S.H. Park, J. Korean Med. Sci. 28 (2013) 593.

[34]        P.A. Humphrey, L.M. Gangarosa, A.J. Wong, G.E. Archer, M. Lund-Johansen, R. Bjerkvig, O.D. Laerum, H.S. Friedman, D.D. Bigner, Biochem. Biophys. Res. Commun. 178 (1991) 1413.

[35]       L.A. Byers, L. Diao, J. Wang,  P. Saintigny, L. Girard, M. Peyton, L. Shen, Y. Fan, U. Giri, P.K. Tumula, M.B. Nilsson, J. Gudikote, H. Tran, R.J. Cardnell, D.J. Bearss, S.L. Warner, J.M. Foulks, S.B.Kanner, V. Gandhi, N. Krett, S.T. Rosen, E.S. Kim, R.S. Herbst, G.R. Blumenschein, J.J. Lee, S.M. Lippman, K.K. Ang, G.B. Mills, W.K. Hong, J.N. Weinstein, I.I. Wistuba,  K.R. Coombes, J.D. Minna, J.V. Heymach, Clin. Cancer Res. 19 (2013) 279.

[36]       J.G. Paez, P.A. Jänne, J.C. Lee, S. Tracy, H. Greulich,   S.  Gabriel,   P.   Herman,  F.J.  Kaye,   N. Lindeman, T.J. Boggon, K. Naoki, H. Sasaki, Y. Fujii, M.J. Eck, W.R. Sellers, B.E. Johnson, M. Meyerson, Science 304 (2004) 1497.

[37]       K. Erjala, M. Sundvall, T.T. Junttila, N. Zhang, M. Savisalo, P. Mali, J. Kulmala, J. Pulkkinen, R. Grenman, K. Elenius, Clin Cancer Res. 12 (2006) 4103.