Modulation of Fourier Transform Infrared Spectra and Copper Levels by Purslane (Portulaca Oleracea) Against Liver Necrosis Induced by Copper Sulphate

Document Type: Article

Authors

1 university of Jiroft

2 University of Jiroft

3 Payam-e-Noor University of Taft

Abstract

Copper (Cu) is an essential trace element involved in normal reproduction but its over-exposure may produce some detrimental effects. The aim of this study was to investigate the effects of purslane on copper sulfate poisoning on liver structures changes. Twenty eight wistar rats were randomly allocated to four treatment groups. Group I) Control, Group II) Copper sulfate (200 mg/kg bw were applied by gavage daily for 4 wk, Group III) Purslane (gavage 400 mg/kg bw daily for 4wk) and Group IV) Combined treatment of copper sulfate and purslane as described in Group II and III. Animals were sacrificed after four weeks and the liver were removed for atomic absorption and Fourier transform infrared spectroscopy (FTIR). A significant increase in the levels of copper and liver weight of copper-treated rats was observed which, however, got moderated significantly upon purslane treatment. The FTIR spectra of the livers of copper-treated rats revealed a significant decrease in the lipid, protein and nucleic acids contents. Treatments with purslane appreciably restored lipids levels, protein as well as nucleic acid contents in the livers of copper-treated rats. The present study suggested that purslane have promising strategy to protect tissue from oxidative stress.

Graphical Abstract

Modulation of Fourier Transform Infrared Spectra and Copper Levels by Purslane (Portulaca Oleracea) Against Liver Necrosis Induced by Copper Sulphate

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