Document Type : Article
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, Academic center for education, culture, and research, Tehran, Iran
Department of Biostatistics, Faculty of Medical Sciences,Tarbiat Modares University, Tehran, Iran
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
It is well known that many biomolecules are chiral and their structure can be monitored by chiroptical spectroscopy. Nevertheless, their analysis in the natural environment of biofluids remains challenging. Recently, little efforts have been made to study biofluids by chiroptical techniques and find a specific signature for healthy/diseased conditions. Blood plasma can be a great subject for this line of examinations. In this study real clinical human blood plasma samples from 46 subjects were analyzed using CD spectropolarimetry to discriminate between type 2 diabetic patients and the control group. For more a challenging condition, patients were selected with type 2 diabetic and ulcer. The results showed a significant decrease in the total α-helical conformation of plasmatic proteins obtained from patients. Also total plasmatic protein content varied with disease condition. As a result, circular dichroism was useful to discriminate between the diabetic patients and the control group, and even between the diabetic patients with or without ulcer. Results of this study encourage possibility of using this technique as a useful supportive tool to conventional diagnostic methods.