Document Type : Article
Molecular Biotechnology Laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz 71454, Iran.
Molecular Biotechnology Laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz 71454, Iran
Natural products have historically been considered as a rich source of therapeutic agents and play a pivotal role in the development of medicinal compounds. Among microorganisms, marine bacteria have been recognized as a rich, promising and untapped resource. In the present work, we focused on Gram-positive bacterial population of Iranian coastal area of the Persian Gulf and study on bioactive potentials of the organic extracts from the isolates. The disc diffusion, DNA-interaction analysis along with MTT assay, and red blood cell hemolysis analysis were performed to evaluate antibacterial, cytotoxicity and anti-inflammatory activities of the extracts. We succeed to identify 6, 39, 34 and 21 novel bacterial producers of the bioactive metabolite having antibacterial, DNA interaction ability, cytotoxic activity, and anti-inflammatory effects, respectively. Approximately 15.4% of the isolates produced antibacterial agents against S. aureus and P. aeruginosa, meanwhile the Bacillus genus revealed the strongest potential. Moreover, a significant correlation was observed between DNA binding ability of the extracts and their cytotoxicity effect on cancerous cell lines; although the chemical nature of the solvent used effects on it. The data of anti-inflammatory study showed that some bacterial extractions are more potent than dexamethasone by more than 90% RBC hemolysis protection. Our results demonstrate that the Gram-positive bacterial population of the Persian Gulf can be considered as a novel rich source of bioactive compounds with valuable therapeutic impact.