Apoptosis Induction by Calystegia Sepium HPLC Fraction can be via Dual Targeting of MDM2 and BCL-2 in Cancer Cells

Document Type: Article

Authors

1 Ph.D., Plant Biotechnology Dep., Faculty of Agricultural sciences, University of Guilan, Rasht, 4199613776, Iran

2 Ph.D., Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran

Abstract

In this research the different extracts of plants were tested to find the special fraction of optimum extract that induce the apoptosis. Cytotoxicity of Methanolic leaf extracts of Catharanthus roseus, Calystegia sepium, Ethanolic leaf extract of Tradescantia pallida and Nasturtium officinale and the Ethanolic and Methanolic bulb extract of Narcissus tazetta treatment after 24 h exposure was investigated using MTT assay and flow cytometric cell cycle and apoptosis tests in HeLa and G292 cancer cells and HGF-1 normal cell. The most effective extract was separated into fractions by preparative HPLC. Fractions cytotoxicity and their effects on some genes expression involved in apoptosis were determined by Real-Time PCR. Results indicated that C5 fraction of Calystegia sepium leaf Methanolic extract created the strong cytotoxic effect on cancer cells when compared to doxorubicin as the standard drugs. In addition, C5 fraction increased the expression level of inflammatory transcription factor like nuclear-factor-KB1, cell cycle inhibitors like TP53 dependent G2 arrest mediator (RPRM) and Cyclin dependent kinase inhibitor 1A (CDKN1A, p21). In contrast, C5 fraction decreased the expression level of MDM2 and BCL-2 that dual targeting of both can consider as a therapeutic strategy. Thus, Calystegia sepium has a lot of potential to be introduced as a new anticancer plant.

Graphical Abstract

Apoptosis Induction by Calystegia Sepium HPLC Fraction can be via Dual Targeting of MDM2 and BCL-2 in Cancer Cells

Keywords