Abstract
The digestive system of hemipteran was adapted to extra-oral digestion. Salivary proteins were injected into the plant cells and after changing form to liquid were transferred into the midgut to complete digestion process. The salivary gland and its secretions have an important function in the feeding process in the insect world. In this work, protein patterns of salivary glands were determined using bottom-up proteomics. Using two-dimensional electrophoresis, gel analysis, gel digestion, liquid chromatography, mass spectrometry, and blast in the specific databases were performed, respectively. Finally, 49 spots were sequenced. Identified proteins classified their physiological roles into seven categories; carbohydrates metabolism, lipid metabolism, protein metabolism, nucleic acid metabolism, defense proteins, structure proteins, and the other groups. Identification of salivary proteins increases our knowledge about the physiology of salivary glands, but also, it can be used in protein engineering to produce new inhibitors that can disrupt insect-plant interaction that lead to decreasing in nutrition in the insect pest. Also using new techniques like proteomics can be considered a great step in protein studies because it facilitates to study of a proteome instead of a protein.
Keywords: Inhibitor; Extra oral digestion; Pest management; Hemiptera; Mass spectrometry; Gel base approach.