TY - JOUR ID - 44263 TI - Spectroscopic Studies on the Interaction of Gold Nanoparticles with Lysozyme JO - Biomacromolecular Journal JA - BMMJ LA - en SN - 7280-2423 AU - Mohammadi, Soraya AU - Khajeh, Khosro AU - Taghdir, Majid AU - Farahani, Nasrin AU - Ranjbar, Bijan AD - Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran AD - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran AD - Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran; AD - Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran AD - Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran Y1 - 2019 PY - 2019 VL - 5 IS - 2 SP - 140 EP - 151 KW - Gold nanoparticles KW - Molten globule-like state KW - Lysozyme KW - Circular dichroism DO - N2 - Gold nanoparticles are promising materials for biomedical applications because of the attractive optical properties such as the absorption and scattering of light at resonant wavelength. Due to the fruitful applications of gold nanoparticles (GNPs), they are appropriate for a variety of biological studies. One of the most important applications of nanoparticles is protein carriers, in which transport of therapeutically relevant protein is done to in vivo or in vitro targets. Protein folding and properties of probable intermediates during the folding of proteins had been investigated in several studies. The molten globule state, a main intermediate of protein folding, has native-like secondary but perturbation of tertiary structure. Consequently, the influence of gold nanoparticles concentration on a model protein was studied by far- and near-UV circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), intrinsic fluorescence emission spectroscopy and 8-Anilino-1-naphthalenesulfonic acid binding. The results indicated that the interactions between gold nanoparticles and lysozyme lead to the formation of molten globule-like state. UR - https://www.bmmj.org/article_44263.html L1 - https://www.bmmj.org/article_44263_a2f7b8999e74eb197628a5dda81aa20a.pdf ER -