Synthetic and Positive Constructs: A New Approach in Molecular Detection

Document Type : Review


1 Department of Bioscience and Biotechnology, Mallek Ashtar University of Technology, Tehran, Iran

2 Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Iran.


Genome-based and molecular identification of pathogens is a common and standard method for various microorganisms that are critical for controlling human diseases. In this field, preparation of standard genome/sequence is very important for setting up experiments and using it for molecular detection. For this prospect, microbial enrichment using its culture is necessary and for the most dangerous pathogens, laboratories with a high level of biological safety are very essential. Furthermore, the lack of access to some pathogenic genomes or standard strains in many countries is a major challenge for an accurate diagnosis. One intelligent and scientific strategy to solve this problem is using synthetic or artificial positive control constructs, which are utilized to ensure designed primers, probes, signal amplification and other characters of reaction works. This study reviews the design and development of positive control constructs for accurate and standard detection of dangerous pathogens for use in the manufacture and development of molecular diagnostic kits.