Document Type : Article
Department of Nanotechnology and Advanced Materials, Materials and Energy Research Centre, Karaj, Iran
Extracellular vesicles (EVs) are natural phospholipid molecular biological compounds endogenously secreted by different cell types and suitable to deliver macromolecules such as proteins. They maintain the physiological balance by mediating the intercellular signaling. Meanwhile, their regenerative potential is mainly related to the regulation of cell apoptosis, differentiation, cell proliferation, inflammation, and angiogenesis. The objective of this study was to evaluate the effects of human Amniotic fluid-derived EVs (hAF‑EVs) on the proliferation of human PC12 (hPC12) cell lines. The EVs from amniotic fluid origin were isolated via differential centrifugation and characterized for morphology and size. Further, the cell proliferation of EVs was examined on the human PC12 cell line at different concentrations (103-1030 nM) in three days. The MTT results revealed that treatment with hAF‑EVs significantly improved the in-vitro cell proliferation. The hAF‑EVs improvement of cell proliferation seemed to be mainly dose dependent. The hAF‑EVs also upregulated the Nrf2 (nuclear factor erythroid 2–related factor 2) expression and reduced the IL-6 (Interleukin 6) expression. The optimizing volume of hAF‑EVs was shown an appropriate treatment possibility in chick embryos with growth retardation.